第1474回生物科学セミナー
The dynamic ECM: from fly development to fibrotic disease
Professor Brian Stramer（Randall Centre for Cell & Molecular Biophysics, King’s College London）
2023年12月05日(火) 14:00-15:30 理学部2号館223号室及びZoom
My lab has been exploiting the embryonic migration of Drosophila macrophages (hemocytes) as a genetically tractable model to understand the role and regulation of cell migration. However, we recently elucidated the surprising function of hemocytes during fly development: the deposition of extracellular matrix (ECM). I will introduce some new projects in the lab focused on understanding the formation, function, and surprising dynamics of the ECM during growth and morphogenesis of Drosophila tissues.
We have also become interested in understanding the regulation of ECM patterning during scarring. ECM architecture in virtually all types of scarring pathologies becomes highly bundled and aligned over supracellular length scales. We have recently shown that patient fibroblasts isolated from keloid scars maintain a unique capacity to generate an aligned ECM network in 2-D culture and I will discuss the cell and molecular mechanisms driving this long-range ECM anisotropy.
参考文献:
Kenny F, Marcotti S, de Freitas D, Drudi E, Leech V, Bell R, Easton J, Diaz de la Loza M, Fleck R, Allison L, Philippeos C, Manhart A, Shaw T, Stramer B. (2023) Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts. Matrix Biol. In Press. DOI: 10.1016/j.matbio.2023.08.004
Serna-Morales E, Sanchez-Sanchez B, Marcotti S, Bhargava A, Hirvonen L, Dragu A, Diaz-de-la-Loza M, Mink M, Cox S, Rayfield E, Stramer B. (2023) Extracellular matrix assembly stress drives Drosophila central nervous system morphogenesis. Dev Cell. 22;58(10):825-835. DOI: 10.1016/j.devcel.2023.03.019
Marcotti S, de Freitas DB, Troughton L, Kenny F, Shaw T, Stramer B, Oakes P. (2021) A workflow for rapid unbiased quantification of fibrillar feature alignment in biological images. Front. Comput. Sci. 3:745831. DOI: 10.3389/fcomp.2021.745831
Marcotti S, Sanchez-Sanchez B, Serna-Morales E, Dragu A, Diaz-de-la-Loza M, Matsubayashi Y, Stramer B. (2021) Protocol for intervention-free quantification of protein turnover rate by steady-state modeling. STAR Protoc. 18;2(1):100377. DOI: 10.1016/j.xpro.2021.100377
Matsubayashi Y, Sanchez-Sanchez B, Marcotti S, Serna-Morales E, Dragu A, Diaz-de-la-Loza M, Vizcay-Barrena G, Fleck R, Stramer B. (2020) Rapid homeostatic turnover of embryonic ECM during tissue morphogenesis. Dev. Cell 6;54(1)33-42. DOI: 10.1016/j.devcel.2020.06.005
Matsubayashi Y, Louani A, Dragu A, Sanchez-Sanchez B, Serna-Morales E, Yolland L, Gyoergy A, Vizcay G, Fleck R, Heddleston J, Chew T, Siekhaus D, Stramer B. (2017) A Moving source of matrix components is essential for de novo basement membrane formation. Curr. Biol. 27(22):3526-3534. DOI: 10.1016.j.cub.2017.10.001
担当： 東京大学大学院理学系研究科・生物科学専攻・多細胞秩序形成学研究室
