第1252回生物科学セミナー
Function of human stress-granule-localizing mRNA-binding protein families
Professor Thomas Tuschl（The Rockefeller University）
2019年02月05日(火) 17:00-18:00 理学部1号館東棟 287号室
RNA-binding proteins and noncoding RNAs coordinate RNA processing and
post-transcriptional gene regulation. We are studying the function of nucleo-cytoplasmic shuttling mRNA-binding proteins (mRBPs) characterized by co-localization to cytoplasmic stress granules (SGs) using CRISPR gene knockout, RNA-seq, proteomic analysis, and Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP). TIA1 family mRBPs are one of the most prominent marker proteins of SGs. Simultaneous loss of both members unexpectedly induced their formation as a consequence of aberrant RNA processing of mRNAs leading to complete loss of the dsRNA binding protein PRKRA and downstream activation of dsRNA-induced protein kinase EIF2AK2.
