濡木研公開ラボセミナー

Drosophila Tool Kit for Studying the Function of Aminoacyl-tRNA Synthetases and Multi-synthetase Complex

Kyu-Sun Lee博士(Metabolism & Neurophysiology Laboratory)

2018年01月18日(Thu)    14:00-14:30  理学部3号館 326号室   

Aminoacyl-tRNA synthetases (ARSs) ligate amino acids to cognate tRNAs by decoding the triplet code during translation. ARS also perform several non-canonical functions in transcription, translation, apoptosis, angiogenesis and inflammation. The multi-synthetase complex (MSC), consisting of nine different ARSs and three scaffold proteins (AIMP1-3), serves as a molecular hub for the role of MSC components in various signaling pathways. However, the assembly process and its biological functions of the MSC are not well understood. The genome of Drosophila contains 19 cytoplasmic ARS to mediate aminoacylation of 20 tRNAs including glutamyl-prolyl-tRNA synthetase (EPRS). In addition, the purified MSC contains the same nine ARSs and three scaffolds proteins both in Drosophila and mammals. These previous reports supported that Drosophila model has become a preferred system to investigate the function of ARS and MSC, including translation, non-canonical activities, and the fidelity of aminoacylation. I will review the strategies and genetic tool kits that are available to dissect the function of ARS and MSC in Drosophila model system. In particular, I will introduce gene replacement technique that induces MSC disruption by selectively removing the domain of MSC components. Also I will briefly present the role of asparaginyl tRNA synthetase (NARS) in tumorigenesis. Taken together, these results provide information and resources for those interested in pursuing Drosophila models of ARS and MSC biology.