第1507回生物科学セミナー
Time-resolved spatial transcriptomes and gene regulatory atlas of Medicago nodule organogenesis
Min-Yao Jhu, Ph.D.（Crop Science Centre, Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom）
2024年06月11日(火) 16:50-18:35 理学部2号館223号室及びZoom
Exploring nodule organogenesis at the spatial-temporal level is pivotal for advancing developmental biology. This understanding opens avenues for integrating beneficial traits into crops while avoiding adverse effects through precise genetic engineering, such as transferring nitrogen-fixing capabilities to non-nodulating species for crop improvement. However, studying the complexity of gene regulatory networks within native spatial tissue poses significant challenges. Furthermore, adapting spatial transcriptome technologies from animal to plant tissues poses substantial hurdles, primarily stemming from the heterogeneous nature of plant cell walls and large vacuoles in plant cells. In response, we employed Xenium in situ, a highly multiplexed fluorescence in situ sequencing technique, offering analysis of spatial gene expression with subcellular precision, high sensitivity, and an exceptional signal-to-noise ratio in Formalin-Fixed Paraffin-Embedded (FFPE) sections. This application enabled the examination of 50 genes and cell-type specific markers within Medicago nodules at 14 and 28 days post-inoculation. Our technique distinctly mapped primary tissue layers and cell types, significantly improving the spatial resolution of marker genes previously identified in single-cell RNA-sequencing datasets of complex plant tissues. Xenium in situ transcends the limitations of traditional genetics and bulk RNA sequencing, offering profound insights into gene expression at the cellular level within intact tissues. This advancement not only fosters a deeper understanding of plant development but also sets a foundation for precise spatial-temporal genetic engineering, paving the way for the development of crops with improved resilience and productivity.
参考文献
Lee, Tak, et al. "Light-sensitive short hypocotyl genes confer symbiotic nodule identity in the legume Medicago truncatula." Current Biology 34.4 (2024): 825-840.
Jhu, Min-Yao, and Giles ED Oldroyd. "Dancing to a different tune, can we switch from chemical to biological nitrogen fixation for sustainable food security?." PLoS Biology 21.3 (2023): e3001982.
Schiessl, Katharina, et al. "NODULE INCEPTION recruits the lateral root developmental program for symbiotic nodule organogenesis in Medicago truncatula." Current Biology 29.21 (2019): 3657-3668.
担当：東京大学大学院理学系研究科・生物科学専攻・発生進化研究室
