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研究業績〜1998年以降の原著論文及び総説〜



ミツバチの社会行動を規定する分子基盤の研究

Iino S, Shiota Y, Nishimura M, Asada M*, Ono, M* and Kubo T*. (2020) Neural activity mapping of bumble bee (Bombus ignitus) brains during foraging flight using immediate early genes. Sci. Rep. in press.
*: corresponding authors

Kumagai H†, Kunieda T†, Nakamura K†, Matsumura Y†, Namiki M, Kohno H, Kubo T. (2020) Developmental stage-specific distribution and phosphorylation of Mblk-1, a transcription factor involved in ecdysteroid-signaling in the honey bee brain. Sci. Rep. in press.
†: Equal contribution

Kohno, H.*, Kubo, T.* (2019) Genetics in the Honey Bee: Achievements and Prospects toward the Functional Analysis of Molecular and Neural Mechanisms Underlying Social Behaviors. Insects 10, 348.
*: corresponding authors

Suenami S*, Miyazaki R and Kubo T. (2018) Detection of phospholipase C activity in the brain homogenate from the honeybee. J. Vis. Exp. (2018)
http://www.jove.com/video/58173
*: corresponding author.

Suenami, S.†, Oya, S.†, Kohno, H.† and Kubo, T. (2018) Kenyon cell subtypes/populations in the honey bee mushroom bodies: possible function based on their gene expression profiles, differentiation, possible evolution, and application of genome editing. Mini Review, Front. Psychol. - Comparative Psychology (Edited by: Prof. Martin Giulfa) in press.
†: equal contribution.

Kohno, H. and Kubo T. (2018) mKast is dispensable for normal development and sexual maturation of the male European honeybee. Sci. Rep. 8(1):11877
(ニュース:ゲノム編集と人工授精を用いたヘテロ変異体ミツバチの作出に世界で初めて成功)
*本論文は、理学部Youtubeチャンネル「研究室の扉」でも紹介されています。
http://www.youtube.com/UTokyoScience
https://youtu.be/sVgrfXSKdVM
*本論文はUTokyoFOCUSに取り上げていただきました。
 ヘテロ変異体ミツバチの作出に世界で初めて成功
 社会性関連遺伝子の機能解析に向けて前進
https://www.u-tokyo.ac.jp/focus/ja/articles/z0508_00044.html

Fujita, T., Kozuka-Hata, H., Hori, Y., Takeuchi, J., Kubo, T., Oyama, M. Shotgun proteomics deciphered age/division of labor-related functional specification of three honeybee (Apis mellifera L.) exocrine glands. PLoS ONE in press (2018)

Suenami S, Iino S, Kubo T. Pharmacologic inhibition of phospholipase C in the brain attenuates early memory formation in the honeybee (Apis mellifera). Biology Open (in press).

Oya S, Kohno H, Kainoh Y, Ono M, Kubo T. (2017) Increased complexity of mushroom body Kenyon cell subtypes in the brain is associated with behavioral evolution in hymenopteran insects. Sci. Rep. 7, 13785
(プレスリリース:ハチ目昆虫の3段階の行動進化と相関する脳高次中枢の進化を解明)

河野大輝、佐々木哲彦、久保健雄 ミツバチにおけるゲノム編集法の開発:その先の眺望 昆虫と自然 52, 37-39 (2017)

Yamane A, Kohno H, Ikeda T, Kaneko K, Ugajin A, Fujita T, Kunieda T, Kubo T. (2017) Gene expansion and immunohistpchemical analyses of mast suggest its late pupal and adult-specific functions in the honeybee brain. PLoS ONE 12(5): e0176809.

Takayanagi-Kiya S*, Kiya T, Kunieda T and Kubo T. (2017) Mblk-1 Transcription factor family: its roles in various animals and regulation by NOL4 splice variants in mammals. Int. J. Mol. Sci. 18, 246
*: corresponding author.

Kohno H, Suenami S, Takeuchi T, Sasaki T and Kubo T. (2016) Production of knockout mutants by CRISPR/Cas9 in the European honeybee, Apis mellifera L. Zool. Sci. 33(5), 505-512.
*本論文は、日本動物学会の「2017年度論文賞」を受賞しました。

Ueno T, Kawasaki K, Kubo T. (2016) Single-cohort colonies and hormone treatment of worker honeybees to analyze physiology associated with role and/or endocrine system. J Visualized Exp http://www.jove.com/video/54240

Kaneko K, Suenami S, Kubo T. (2016) Gene expression profiles and neural activities of Kenyon cell subtypes in the honeybee brain: Identification of novel ‘middle-type’ Kenyon cells (review). Zool. Lett. 2:14

Suenami S, Paul RK, Takeuchi H, Okude G, Fujiyuki T, Shirai K and Kubo T. (2016) Analysis of the differentiation of Kenyon cell subtypes using three mushroom body-preferential genes during metamorphosis in the honeybee (Apis mellifera L.). PLoS ONE 11(6): e0157841

Ueno, T., Takeuchi, H., Kawsaki, K., and Kubo, T. (2015) Changes in the gene expression profiles of the hypopharyngeal gland of worker honeybees in association with worker behavior and hormonal factors. PLoS ONE 10(6): e0130206.

Watanabe T, Kubo T. A new antigenic marker specifically labels a subpopulation of the class II Kenyon cells in the brain of the European honeybee Apis mellifera. Biophysics 11, 73-77 (2015)

Takayanagi-Kiya S, Misawa-Hojo K, Kiya T, Kunieda T, Kubo T (2014) Splicing variants of NOL4 differentially regulate the transcription activity of Mlr1 and Mlr2 in cultured cells. Zoological Science 31, 735-740.

久保健雄、奥山輝大、上川内あづさ、竹内秀明/共著;太田次郎、赤坂甲治、浅島誠、長田敏行/編集 第6章 社会性昆虫ミツバチの行動分子生物学 新・生命科学シリーズ 動物行動の分子生物学 裳華房 pp. 130-174 (2014)
関連記事:理学部ニュース 理学の本棚 第14回

金子九美、久保健雄 ミツバチの「尻振りダンス」の分子・神経的基盤の解析 生化学 86, 589-594 (2014)

Ugajin, A., Kunieda, T., Kubo, T. (2013) Identification and characterization of an Egr ortholog as a neural immediate early gene in the European honeybee (Apis mellifera L.). FEBS Lett. 587, 3224.

Kaneko, K., Ikeda, T.*, Nagai, M.*, Hori, S.*, Umatani, C., Tadano, H., Ugajin, A., Nakakoka, T., Paul, R. K., Fujiyuki, T., Shirai, K., Kunieda, K., Takeuchi, H. and Kubo, T. (2013) Novel middle-type Kenyon cells in the honeybee brain revealed by area-preferential gene expression analysis. PLoS ONE 8, e71732.
(プレスリリース:採餌飛行をするミツバチの脳で活動する新規な神経細胞「中間型ケニヨン細胞」の発見)

Shiota, Y. and Kubo, T. (2013) Ecdysteroids and Honeybee Social Bheviors. In: Hormones and Behavior (Ed: Simonsen, D.) eBook, NOVA Science Publishers., 135-156.

Taketoshi Kiya *, Atsushi Ugajin, Takekazu Kunieda, Takeo Kubo (2012) Identification of kakusei, a nuclear non-coding RNA, as an immediate early gene from the honeybee, and its application for neuroethological study Intl. J. Mol. Sci. 13(12), 15496-15509.

Fujita, T., Kozuka-Hata, H., Ao-Kondo, H., Kunieda, T., Oyama, M. and Kubo, T. (2012) Proteomic analysis of the royal jelly and characterization of the functions of its derivation glands in the honeybee. J. Proteomic Res. 12(1), 404-411.

Uno, Y., Fujiyuki, T., Morioka, M and Kubo, T. (2012) Mushroom body-preferential expression of proteins/genes involved in endoplasmic reticulum Ca2+-transport in the worker honeybee (Apis mellifera L.) brain. Insect Mol.Biol. 22(1), 52-61.

Ugajin, A., Kiya, T., Kunieda, T., Ono, M., Yoshida, T. and Kubo, T. (2012) Detection of neural activity in the brains of Japanese honeybee workers during the formation of a “hot defensive bee ball” PLoS ONE 7(3), e32902.
(プレスリリース:ニホンミツバチの攻撃行動(熱殺蜂球形成)における脳の活動を解明)

Kubo, T. (2012) Neuroanatomical dissection of the honeybee brain based on temporal and regional gene expression patterns. In: Honeybee neurobiology and Behavior. A Tribute to Randolf Menzel (eds. Galizia CG, Eisenhardt D and Giulfa M). Springer, .

Kiya, T.† and Kubo, T. (2011) Dance type and flight parameters are associated with different mushroom body neural activities in worker honeybee brains. PLoS ONE 6(4), e19301.
†: corresponding author

Yamazaki, Y., Kiuchi, M., Takeuchi, H. and Kubo, T. (2011) Ecdysteroid biosynthesis in workers of the European honeybee (Apis mellifera L.). Insect Biochem. Mol. Biol. 41, 283-293.

Fujita, T., Kozuka-Hata, H., Uno, Y., Nishikori, K., Morioka, M., Oyama, M. and Kubo, T. (2010) Functional analysis of the honeybee (Apis mellifera L.) salivary system using proteomics. Biochem. Biophys. Res. Commun. 397, 740-744.

Kaneko, K., Hori, S.*, Morimoto, M. M.*, Nakaoka, T., Paul, R. K., Fujiyuki, T., Shirai, K., Wakamoto, K., Tsuboko, S., Takeuchi, H. and Kubo, T. (2010) In situ hybridization analysis of the expression of futsch, tau, and MESK2 homologues in the brain of the European honeybee (Apis mellifera L.). PLoS ONE 5(2), e9213.
*: equal contribution

Watanabe, T.*, Takeuchi, H. and Kubo, T.* (2010) Structural diversity and evolution of the N-terminal isoform-specific region of ecdysone receptor-A and -B1 isoforms in insects. BMC Evol. Biol. 10, 40.
*: corresponding authors

Hori, S.*†, Kaneko K.*, Saito, T., Takeuchi, H. and Kubo, T. (2010) Expression of two microRNAs, ame-mir-276 and -1000, in the adult honeybee (Apis mellifera) brain. Apidologie 42, 89-102.
†:corresponding author, *: equal contribution

Kiya, T.* and Kubo, T. (2010) Analysis of GABAergic and non-GABAergic neuron activity in the optic lobes of the forager and re-orienting worker honeybee (Apis mellifera L.). PLoS ONE 5(1), e8833.
*: corresponding author

Hirotsu T, Hayashi Y, Iwata R, Kunitomo H, Kage-Nakadai E, Kubo T, Ishihara T and Iino Y. Behavioural assay for olfactory plasticity in C. elegans. Nat. Protocols 139 (2009)

Tomoko Fujiyuki*, Emiko Matsuzaka, Takayoshi Nakaoka, Hideaki Takeuchi, Akiko Wakamoto, Seii Ohka, Kazuhisa Sekimizu, Akio Nomoto, and Takeo Kubo (2009) Distribution of Kakugo virus and its effects on the gene expression profile in the brain of the worker honeybee Apis mellifera L. J. Virol. 83(22), 11560-11568.
*: corresponding author

Makoto Hasegawa*, Satomi Asanuma*, Tomoko Fujiyuki*, Taketoshi Kiya*, Tetsuhiko Sasaki, Daisuke Endo, Mizue Morioka† and Takeo Kubo (2009) Differential gene expression in the mandibular glands of queen and worker honeybees, Apis mellifera L.: Implication for caste-selective aldehyde and fatty acid metabolism. Insect Biochem. Mol. Biol. 39, 661-667.
*: equal contribution †: corresponding author

Ueno, T., Nakaoka, T., Takeuchi, H.*, and Kubo, T.* (2009) Differential gene expressions in the hypopharyngeal glands of worker honeybees (Apis mellifera L.) associated with the age-dependent role change. Zoological Science 26(8), 557-563.
*: corresponding authors

Hiroto Tadano, Yurika Yamazaki, Hideaki Takeuchi*, and Takeo Kubo (2009) Differential expression associated with age-dependent division of labor and neural subtype-specific expression of a novel non-coding RNA, Nb-1, in the honeybee (Apis mellifera L.) worker brain. Insect Mol.Biol. 18(6), 715-726.
*: corresponding author

Hayashi, Y.†, Hirotsu, T., Iwata, R., Kage-Nakadai, E., Kunitomo, H., Ishihara, T., Iino Y. and Kubo, T. (2009) A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans. Nature Neurosci. 12, 981-987.
†: corresponding author
(プレスリリース:脳の配線ミスを生後の発達期に修正する新たなメカニズムの発見)

藤幸知子、久保健雄 (2008) 『攻撃的なミツバチから見つかったKakugoウイルスの疫学的調査』  化学と生物 46, 491-495.

木矢剛智、堀沙耶香、竹内秀明、久保健雄 (2008) 『ミツバチの“ダンスコミュニケーション”の神経基盤の理解に向けてー野外と室外における新しい2つの実験系ー』 蛋白質核酸酵素 53, 1368-1374 .

Nakaoka, T., Takeuchi, H.*, and Kubo, T. (2008) Laying workers in queenless honeybee (Apis mellifera L.) colonies have physiological states similar to that of nurse bees but opposite that of foragers. J Insect Physiol. 54, 806-812.
*: corresponding author

Kiya, T.†, Kunieda, T., and Kubo, T.† (2008) Inducible- and constitutive-type transcript variants of kakusei, a novel non-coding immediate early gene, in the honeybee brain. Insect Mol.Biol. 17(5), 531-536.
†: corresponding author

Kiya, T.†, Itoh, Y., and Kubo, T.† (2008) Expression analysis of the FoxP homologue in the brain of the honeybee, Apis mellifera L. Insect Mol.Biol. 17, 53-60.
†: corresponding author

久保健雄 (2007) 『ミツバチ脳のキノコ体(高次中枢)と社会性行動』 脳 21 金芳堂、10(4), 366-370.

久保健雄 (2007) 『ミツバチの社会性行動を規定する分子的基盤』 シリーズ 21世紀の動物科学 第8巻 行動とコミュニケーション (岡良隆・蟻川謙太郎共編)、培風館, pp.8-36.

Hori, S., Takeuchi, H.†, and Kubo, T. (2007) Associative learning and discrimination of motion cues in the harnessed honeybee Apis mellifera L. J. Comp.Physiol. A 193, 825-833.
†: corresponding author

Kiya, T.†, Kunieda, T., and Kubo, T.† (2007) Increased neural activity of a mushroom body neuron subtype in the brains of forager honeybees. PLoS ONE 4, e371.
†: corresponding author

國枝武和、藤幸知子、久保健雄 (2007) 『社会性昆虫ミツバチのゲノムが語るもの-ミツバチのゲノムから社会性の理解へ-』レビュー、 生物の科学・遺伝 61(2), 4-7.

Takeuchi, H.*†, Paul, R. K.*, Matsuzaka, E., and Kubo, T. (2007) EcR-A expression in the brain and ovary of the honeybee (Apis mellifera L.). Zoological Science 24(6), 596-603.
*: equal contribution. †: corresponding author

Uno, Y., Fujiyuki, T., Takeuchi, H., Morioka, M. and Kubo, T. (2007) Identification of proteins whose expression is up- or down-regulated in the mushroom bodies in the honeybee brain using proteomics. FEBS Lett. 581, 97-101.

Hideaki Takeuchi. (2006) Gene expression in the Honeybee Brain Mushroom Body and its Gene Orthologues. In "Evolution of Nervous Systems. Vol1" Ed by Jpn H Kaas, Elsevier, pp 457-469.

Ando, T.*, Fujiyuki, T.*, Kawashima, T.*, Morioka, M., Kubo, T.†, and Fujiwara, H.† (2006) In vivo gene transfer into the honeybee using a nucleopolyhedrovirus vector. Biochem. Biophys. Res. Commun. 352, 335-340 .
*, equal contribution; †, corresponding authors

Fujiyuki,T., Ohka,S., Takeuchi,H., Ono,M., Nomoto,A., and Kubo,T. (2006) Prevalence and phylogeny of Kakugo virus, a novel insect picorna-like virus that infects the honeybee (Apis mellifera L.), under various colony conditions. J. Virol. 80, 11528-11538 .

Paul, R. K.*, Takeuchi, H.* and Kubo, T. (2006) Expression of two ecdysteroid-regulated genes, Broad-Complex and E75, in the brain and ovary of the honeybee (Apis mellifera L.). Zoological Science 23(12), 1085-1092.
*: equal contribution.

Kunieda, T. *, Fujiyuki, T.*, Kucharski, R.*, Foret, S.*, Ament, S. A.*, Toth, A.L.*, Ohashi, K, Takeuchi, H, Kamikouchi, A., Kage, E., Morioka, M., Beye, M.†, Kubo, T.†, Robinson, G.E.†, Maleszka, R.† (2006) Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome. Insect Mol.Biol. 15(5), 563-576.
*, equal contribution; †, corresponding authors

Lehman, H. K., Schulz, D. J., Barron, A. B., S. A., Wraight, L., Hardison, C., Whitney, S., Takeuchi, H., Paul, R. K., and Robinson, G. E. (2006) Division of labor in the honey bee (Apis mellifera): The role of tyramine beta-hydroxylase, J. Exp.Biol. 209, 2774-2784.

Yamazaki, Y*., Shirai, K*., Paul, R. K., Fujiyuki, T., Wakamoto, A., Takeuchi, H., and Kubo, T. (2006) Differential expression of HR38 in the mushroom bodies of the honeybee brain depends on the caste and division of labor. FEBS Lett. 580, 2667-2670.
*: equal contribution

Hori, S.,Takeuchi, H†., Arikawa, K., Kinoshita, M., Ichikawa, N., Sasaki, M., and Kubo, T. (2006) Associative visual learning, color discrimination, and chromatic adaptation in the harnessed honeybee Apis mellifera L. J. Comp.Physiol. A 192, 691-700. †: corresponding author.
本論文はBiology Reports Ltd. の「Faculty of 1000」に重要論文として推薦されました。

國枝武和、久保健雄 (2005) 『ミツバチ脳の高次中枢(キノコ体)選択的に発現する新規転写因子Mblk-1のリン酸化による活性制御』 レビュー、 日本応用酵素協会誌 40, 25-31 .

Fujiyuki, T., Takeuchi, H., Ono, M., Ohka, S., Sasaki, T., Nomoto, A. and Kubo, T. (2005) Review. Kakugo virus from brains of aggressive worker honeybees, Adv. Vir. Res. 65 , 1-27 .

Kage, E*., Hayashi, Y*., Takeuchi, H., Hirotsu, T., Kunitomo, H., Inoue, T., Arai, H., Iino, Y. and Kubo, T. (2005) MBR-1, a novel helix-turn-helix transcription factor, is required for pruning excessive neurites in Caenorhabditis elegans. Curr. Biol. 15, 1554-1559.
*: equal contribution

Paul, K. R*., Takeuchi, H*., Matsuo, Y. and Kubo, T. (2005) Gene expression of ecdysteroid-regulated gene E74 of the honeybee in ovary and brain. Insect Mol.Biol. 14, 9-15 .
*: equal contribution

藤幸知子、竹内秀明、久保健雄 (2004) 『セイヨウミツバチの攻撃的な働き蜂の脳から同定されたKakugoウイルス』」レビュー、 ミツバチ科学 25(4), 145-151.

竹内秀明・國枝武和・徳廣(澤田)美由紀・朴正敏・藤幸知子・久保健雄 (2004) 『ミツバチをモデル生物とした分子行動生物学』」レビュー、 蛋白質核酸酵素 49 (16), 2542-2548.

藤幸知子 (2004) 『ミツバチの攻撃性の研究から Kakugo ウイルスの同定に至るまで』レビュー、 昆虫DNA研究会レター 1 , 38-42 .

Sawata, M., Takeuchi, H. and Kubo, T. (2004) Identification and analysis of the minimal promoter activity of a novel non-coding nuclear RNA gene, AncR-1, from the honeybee (Apis mellifera L.). RNA 10, 1047-1058 .

Kunieda, T. and Kubo, T. (2004) In vivo gene transfer into the adult honeybee brain by using electroporation. Biochem. Biophys. Res. Commun. 318, 25-31 .

Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y.,Sawata, M., Matsuo, Y., Kato, A., Tsujimoto, A., Nakajima, T. and Kubo, T. (2004) Prepro-tachykinin gene expression in the honeybee Apis mellifera L. brain. Cell Tissue Res. 316, 281-293 .

Fujiyuki, T., Takeuchi, H., Ono, H., Ohka, S., Sasaki, T., Nomoto, A., and Kubo, T. (2004) A novel insect picorna-like virus identified in the brains of aggressive worker honeybees. J. Virol. 78, 1093-1100.

Kamikouchi, A., Morioka, M. and Kubo, T. (2004) Identification of honeybee antennal proteins/genes expressed in a sex- and/or caste selective manner. Zoological Science 21, 53-62.

Kubo, T. (2003) Molecular analysis of the honeybee sociality. In “Genes, Behaviors and Evolution of Social Insects (Eds: Kikuchi, T., Azuma, N. and Higashi, S.)” Hokkaido University Press, , pp. 3-20.

Park, J.-M., Kunieda, T., and Kubo, T. (2003) The activity of Mblk-1, a mushroom body-selective transcription factor from the honeybee, is modulated by the Ras/MAPK pathway. J. Biol. Chem. 278, 18689-18694 .

Takeuchi, H*., Yasuda, A*., Yasuda-Kamatani, Y*., Kubo, T. and Nakajima T. (2003) Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen, and drone heads. Insect Mol.Biol. 12, 291-298 .
*: equal contribution

Kunieda, T., Park, J.-M., Takeuchi, H. and Kubo, T. (2003) Identification and characterization of Mbr1, 2: two mouse homologues of Mblk-1, a transcription factor from the honeybee brain. FEBS Lett. 535, 61-65 .

Sawata, M., Yoshino, D., Takeuchi, H., Kamikouchi, A., Ohashi, K. and Kubo, T. (2002) Identification of a novel gene, Ks-1, which encodes a non-coding nuclear RNA expressed in a restricted region of the honeybee brain. RNA 8, 772-785 .

Takeuchi, H., Fujiyuki, T., Shirai, K., Matsuo, Y., Kamikouchi, A., Fujinawa, Y., Kato, A., Tsujimoto, A. and Kubo, T. (2002) Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray. FEBS Lett. 513, 230-234 .

Park, J-M., Kunieda, T., Takeuchi, H., and Kubo, T. (2002) DNA binding properties of Mblk-1, a putative transcription factor from the honeybee. Biochem. Biophys. Res. Commun. 291, 23-28.

Takeuchi, H., Kage, E., Sawata, M., Kamikouchi, A., Ohashi, K., Ohara, M., Fujiyuki, T., Kunieda, T., Sekimizu, K., Natori, S., and Kubo, T. (2001) Identification of a novel gene, Mblk-1, that encodes a putative transcription factor expressed preferentially in the large-type Kenyon cells of the honey bee brain. Insect Mol.Biol. 10, 487-494 .

Kamikouchi, A., Takeuchi, H., Sawata, M., Natori, S., Kubo, T. (2000) Concentrated expression of Ca2+/ calmodulin-dependent protein kinase II and protein kinase C in the mushroom bodies of the brain of the honeybee Apis mellifera L. J. Comp. Neurol. 417, 501-510 .

Ohashi, K., Sasaki, M., Sasagawa, H., Nakamura, J., Natori, S., Kubo, T. (2000) Functional Flexibility of the honeybee hypopharyngeal gland in a dequeened colony. Zoological Science 17, 1089-1094 .

Ohashi, K., Natori, S., Kubo, T. (1999) Expression of amylase and glucose oxidase in the hypopharyngeal gland with an age-dependent role change of the worker honeybee (Apis mellifera L.). Eur. J. Biochem. 265 , 127-133 .

Kamikouchi, A., Takeuchi, H., Sawata, M., Ohashi, K., Natori, S., Kubo, T. (1998) Preferential expression of the gene for a putative inositol 1,4,5-trisphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L. Biochem. Biophys. Res. Commun. 242, 181-186.

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動物の器官再生の分子機構の研究

Kato S, Fukazawa T, Kubo T (2021) Low-temperature incubation improves both knock-in and knock-down efficiencies by the CRISPR/Cas9 system in Xenopus laevis as revealed by quantitative analysis. Biochem. Biophys. Res. Commun. 543, 50-55.

Tsujioka H, Kunieda T, Katou Y, Shirahige K, Fukazawa T, Kubo T (2017) interleukin-11 induces and maintains progenitors of different cell lineages during Xenopus tadpole tail regeneration. Nature Commun. 8, 495. doi: 10.1038/s41467-017-00594-5
(プレスリリース:インターロイキン11は、オタマジャクシの尾再生芽の未分化細胞の誘導・維持に働く—器官再生の最初期段階の人為的再現に成功—)

Hatta-Kobayashi Y, Toyama-Shirai M, Yamanaka T, Takamori M, Wakabayashi Y, Naora Y, Kunieda, Fukazawa T, Kubo T (2016) Acute phase response in amputated tail stumps and neural tissue-preferential expression in tail bud embryos of the Xenopus neuronal pentraxin I gene. Dev. Growth Differ. 58(9), 688-701.

Furusawa Y, Kubo T, Fukazawa T (2016) Phyhd1, an XPhyH-like homologue, is induced in mouse T cells upon T cell stimulation. Biochem. Biophys. Res. Commun. 472(3), 551-556.

Tsujioka H, Kunieda T, Katou Y, Shirahige K, Kubo T (2015) Unique Gene Expression Profile of the Proliferating Xenopus Tadpole Tail Blastema Cells Deciphered by RNA-Sequencing Analysis. PLoS ONE 10(3), e0111655.
DOI: 10.1371/journal.pone.0111655

Naora Y, Hishida Y, Fukazawa T, Kunieda T, Kubo T (2013) Expression analysis of XPhyH-like during development and tail regeneration in Xenopus tadpoles: possible role of XPhyH-like expressing immune cells in impaired tail regenerative ability. Biochem. Biophys. Res. Commun. 431(2), 152-157.
* Global Medical Discovery (http://globalmedicaldiscovery.com/) の重要科学論文(Key Scientific Article) に選ばれました(2013.9.11)

深澤太郎,直良悠子,國枝武和,久保健雄 (2011) 『免疫抑制による“再生不応期”のアフリカツメガエル幼生尾の再生能の人為的活性化:脊椎動物の器官再生能の有無を決定する要因を探る』 Special Review、 細胞工学 30(4), 418-423.

Fukazawa, T., Naora, Y., Kunieda, T. and Kubo, T. (2009) Suppression of the immune response potentiates tadpole tail regeneration during the refractory period. Development 136, 2323-2327.
* 本論文はBiology Reports Ltd. の「Faculty of 1000」で「Must read」との評価をいただきました。

Ishino, T.*, Kunieda, T.*, Natori, S., Sekimizu, K., and Kubo, T. (2007) Identification of novel members of the Xenopus Ca2+-dependent lectin family and analysis of their gene expression during tail regeneration and development. J. Biochem. 141(4), 479-488.
*: equal contribution

Ishino, T., Shirai, M., Kunieda, T., Sekimizu, K., Natori, S. and Kubo, T. (2003) Identification of genes induced in regenerating Xenopus tadpole tails by using the differential display method. Dev. Dyn. 226, 317-325 .

Arai, T., Kubo, T. and Natori, S. (2000) Identification, characterization and cDNA cloning of two novel proteins secreted into the external space of the regenerating leg of Periplaneta americana. Insect Biochem. Mol. Biol. 30, 287-295.

Arai, T., Kawasaki, K., Kubo, T., Natori, S. (1998) Cloning of cDNA for regenectin, a humoral C-type lectin of Periplaneta americana, and expression of the regenectin gene during leg regeneration. Insect Biochem. Mol. Biol. 28, 987-294 .

Kitabayashi-Nomura, A., Arai, T., Kubo, T., Natori, S. (1998) Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana (American cockroach). Insect Biochem. Mol. Biol. 28, 785-790.

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動物(クマムシ)の乾眠・極限環境耐性の分子基盤の研究

Furukawa S, Nagamatsu A, Nenoi M, Fujimori A, Kakinuma S, Katsube T,Wang B, Tsuruoka C, Shirai T, Nakamura AJ, Sakaue-Sawano A, Miyawaki A,Harada H, Kobayashi M, Kobayashi J, Kunieda T, Funayama T, Suzuki M, Miyamoto T, Hidema J, Yoshida Y, Takahashi A (2020) Space Radiation Biology for “Living in Space”. BioMed Res Int 2020, 4703286. doi:10.1155/2020/4703286

Sugiura K, Minato H, Suzuki AC, Arakawa K, Kunieda T, Matsumoto M (2019) Comparison of Sexual Reproductive Behaviors in Two Species of Macrobiotidae (Tardigrada: Eutardigrada). Zool Sci 36(2), 120-127. doi:10.2108/zs180103

Yoshida Y, Koutsovoulos G, Laetsch DR, Stevens L, Kumar S, Horikawa DD, Ishino K, Komine S, Kunieda T, Tomita M, Blaxter M and Arakawa K (2017) Comparative genomics of the tardigrades Hypsibius dujardini and Ramazzottius varieornatus. PLOS Biol 15(7), e2002266. doi:10.1371/journal.pbio.2002266.

Hashimoto T and *Kunieda T (2017) DNA Protection Protein, a Novel Mechanism of Radiation Tolerance: Lessons from Tardigrades. Life 7(2), 26. doi:10.3390/life7020026
*corresponding author

Hashimoto T1, Horikawa DD1, Saito Y, Kuwahara H, Kozuka-Hata H, Shin-I T, Minakuchi Y, Ohishi K, Motoyama A, Aizu T, Enomoto A, Kondo K, Tanaka S, Hara Y, Koshikawa S, Sagara H, Miura T, Yokobori S, Miyagawa K, Suzuki Y, Kubo T, Oyama M, Kohara Y, Fujiyama A, Arakawa K, Katayama T, *Toyoda A and *Kunieda T (2016) Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature Commun 7, 12808. doi: 10.1038/ncomms12808.
1,authors equally contributed. *corresponding author
(プレスリリース:ヒト培養細胞の放射線耐性を向上させる新規タンパク質をクマムシのゲノムから発見)

Ito M1, Saigo T1, Abe W, Kubo T and *Kunieda T1 (2016) Establishment of an isogenic strain of the desiccation-sensitive tardigrade Isohypsibius myrops (Parachela, Eutardigrada) and its life history traits. Zool J Linn Soc 178(4), 863-870. doi: 10.1111/zoj.12449.
1:authors equally contributed. *corresponding author

Kondo K, Kubo T and Kunieda T (2015) Suggested Involvement of PP1/PP2A Activity and De Novo Gene Expression in Anhydrobiotic Survival in a Tardigrade, Hypsibius dujardini, by Chemical Genetic Approach. PLoS ONE 10(12), e0144803. doi: 10.1371/journal.pone.0118272.

Tanaka S, Tanaka J, Miwa Y, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Kubo T, Kunieda T. (2015) Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic tardigrade improve osmotic tolerance of human cells. PLoS ONE 10(2), e0118272.
doi: 10.1371/journal.pone.0118272. PMID: 25675104

伊藤 政博、鳴海 一成、佐藤 孝子、中村 聡、東端 啓貴 、國技 武和、為我井 秀行、道久 則之、伊藤 隆 (2014) 極限環境生命―生命の起源を考え、その多様性に学ぶ , .
コロナ社(12章を担当)

Yamaguchi, A., Tanaka, S., Yamaguchi, S., Kuwahara, H., Takamura, C., Imajoh-Ohmi, S., Horikawa, D.D., Toyoda, A., Katayama, T., Arakawa, K., Fujiyama, A., Kubo, T., Kunieda, T. (2012) Two Novel Heat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic Tardigrade. PLoS ONE 7(8), e44209.
doi:10.1371/journal.pone.0044209 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0044209

國枝武和 (2012) 緩歩動物クマムシの乾眠能力と極限環境耐性 生物科学 63(4), 205-213.

Horikawa DD, Yamaguchi A, Sakashita T, Tanaka D, Hamada N, Yukuhiro F, Kuwahara H, Kunieda T, Watanabe M, Nakahara Y, Wada S, Funayama T, Katagiri C, Higashi S, Yokobori S-I, Kuwabara M, Rothschild L J, Okuda T, Hashimoto H, and Kobayashi Y (2012) Tolerance of Anhydrobiotic Eggs of the Tardigrade Ramazzottius varieornatus to Extreme Environments Astrobiology 12(4), 283-289.

Horikawa, D.D., Kunieda, T., Abe, W., Watanabe, M., Nakahara, Y., Yukuhiro, F., Sakashita, T., Hamada, N., Wada, S., Funayama, T., Katagiri, C., Kobayashi, Y., Higashi, S. and Okuda, T. (2008) Establishment of a Rearing System of the Extremotolerant Tardigrade Ramazzottius varieornatus: A New Model Animal for Astrobiology. Astrobiology 8(3), 1-8.

堀川大樹、國枝武和 (2007) 『高い放射線耐性をもつ動物「クマムシ」』 東京大学アイソトープ総合センターニュース 38(3), 2-4.

國枝武和 (2007) 『第一回クマムシ研究会―不思議な生物とそれに魅せられた人たち―』 科学(岩波書店) 77(4), 338-339.

國枝武和 (2006) 『動物の乾燥耐性を支える分子基盤を探る-クマムシの乾眠の追究からこれまで未解明の耐乾燥戦略の存在が明らかに-』 化学と生物 44(11), 732-734 .

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小型魚類の社会性行動、適応的行動を可能にする脳機能の統合的解析

Yokoi S, Okuyama T, Kamei Y, Naruse K, Taniguchi Y, Ansai S, Kinoshita M, Young LJ, Takemori N, Kubo T, Takeuchi H. (2015) An essential role of the arginine vasotocin system in mate-guarding behaviors in triadic relationships of Medaka fish (Oryzias latipes). PLOS Genetics 11, e1005009

Tsuboko S, Kimura T, Shinya M, Suehiro Y, Okuyama T, Shimada A, Takeda H, Naruse K, Kubo T, Takeuchi H. (2014) Genetic control of startle behavior in medaka fish. PLoS ONE 9, e112527

Okuyama T, Yokoi S, Abe H, Isoe Y, Suehiro Y, Imada H, Tanaka M, Kawasaki T, Yuba S, Taniguchi Y, Kamei Y, Okubo K, Shimada A, Naruse K, Takeda H, Oka Y, Kubo T, Takeuchi H (2014) A neural mechanism underlying mating preferences for familiar individuals in medaka fish. Science 343, 91-94.

竹内秀明 (2014)「メダカを用いた分子遺伝学手法による魚類「社会脳」の分子神経基盤の解明 比較生理生化学 31, 106-112.

Ochiai, T., Suehiro, Y., Nishinari, K., Kubo, T. and Takeuchi, H. (2013) A new data-mining method to search for behavioral properties that induce alignment and their involvement in social learning in medaka fish (Oryzias latipes). PLoS ONE 8, e71685.

Okuyama, T., Isoe, Y., Hoki, M., Suehiro, Y., Yamagishi, G., Naruse, K., Kinoshita M., Kamei, Y., Shimizu, A., Kubo, T. and Takeuchi, H. (2013) Controlled Cre/loxP site-specific recombination in the developing brain in medaka fish, Oryzias latipes. PLoS ONE 8, e66597.

Isoe, Y., Okuyama, T., Taniguchi, Y., Kubo, T. and Takeuchi, H. (2012) p53 mutation suppresses adult neurogenesis in medaka fish (Oryzias latipes) Biochem. Biophys. Res. Commun. 423(4), 627-631.

Okuyama, T., Suehiro, Y., Imada, H., Shimada, A., Naruse, K., Takeda, H., Kubo, T. and Takeuchi, H.† (2011) Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli. Biochem. Biophys. Res. Commun. 404, 453-457.
†: corresponding author

Kuroyanagi, Y.*, Okuyama, T.*, Suehiro, Y., Imada, H., Shimada, A., Naruse, K., Takeda, H., Kubo, T. and Takeuchi, H. (2010) Proliferation zones in the adult medaka (Oryzias latipes) brain. Brain Res. 1323, 33-40.
*: equal contribution

Suehiro, Y., Kinoshita, M.†, Okuyama, T., Shimada, A., Naruse, K., Takeda, H., Kubo, T., Hashimoto, M.†, and Takeuchi, H.† (2010) Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system. FEBS Lett. 584,, 3545-3549.
†: corresponding authors

Imada, H., Hoki, M., Suehiro, Y., Okuyama, T., Kurabayashi, D., Shimada, A., Naruse, K., Takeda, H., Kubo, T., and Takeuchi, H.† (2010) Coordinated and cohesive movement of two small conspecific fish induced by eliciting a simultaneous optomotor response. PLoS ONE 5, e11248.
†: corresponding author

Suehiro, Y.*, Yasuda, A.*, Okuyama, T., Imada, H., Kuroyanagi, Y., Kubo. T. & Takeuchi, H.† (2009) Mass spectrometric map of neuropeptide expression and analysis of the g-prepro-tachykinin gene expression in the medaka (Oryzias latipes) brain. Gen. Comp. Endocrinol. 161, 138-145.
*:equal contribution. †:corresponding author

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細胞内共生の分子生物学的・細胞生物学的な研究

Nishikori K, Morioka K, Kubo T, Morioka M. (2009) Age- and morph-dependent activation of the lysosomal system and Buchnera degradation in aphid endosymbiosis. J Insect Physiol. 55(4), 351-357.

Nishikori K, Kubo T, Morioka M. (2009) Morph-dependent expression and subcellular localization of host serine carboxypeptidase in bacteriocytes of pea aphid associated with degradation of the endosymbiotic bacterium, Buchnera. Zoological Science 26(6), 415-420.

Maezawa, K., Shigenobu, S., Taniguchi, H., Kubo, T., Aizawa, S. and Morioka, M. (2006) Hundreds of flagellar basal bodies cover the cell surface of the endosymbiotic bacterium Buchnera aphidicola sp. strain APS. J. Bacteriol. 188, 6539-6543 .

Sasaki, T., Massaki, N., Kubo, T. (2005) Wolbachia variant that induces two distinct reproductive phenotypes in different hosts. Heredity 95, 389-393 .

Hojo, M., Morika, M., Matsumoto, M., and Miura, T. (2005) Identification of soldier caste-specific protein in the frontal gland of nasute termite Nasutitermes takasagoensis (Isoptera: Termitidae). Insect Biochem. Mol. Biol. 35, 347-354 .

Kutsukake, M.,Shibao, H., Nikoh, N., Morioka, M.,Tamura, T., Hishino, T., Phgiya, S. and Fukatsu, T. (2004) Venomous protease of aphid soldier for colony defense. Proc. Natl. Acad. Sci. USA. 101, 11338-11343.

Fujii, Y., Kubo, T., Ishikawa, H. and Sasaki, T. (2004) Isolation and characterization of the bacteriophage WO from Wolbachia, an arthropod endosymbiont. Biochem. Biophys. Res. Commun. 317(4), 1183-1188.

Tomoyasu, T., Takaya, T., Nagase, T., Kikuno, R., Morioka, M., and Yamamoto, T. (2003) A new heat shock gene, agsA, which encodes a small chaperone involved in suppressing protein aggregation in Salmonella enterica serovar Typhimurium. J. Bacteriol. 185, 6331-6339.

Yamaguchi, Y., Tomoyasu, T., Takaya, A., Morioka, M., and Yamamoto, Y. (2003) Effects of disruption of heat shock genes on susceptibity of Escherichia coli to fluoroquinolones. BMC Microbiology 3, 16.

Unno, K., Kishido, T., Morioka, M., and Oku, N. (2003) Increased expression of Hsp70 for resistance to deuterium oxide in a yeast mutant cell line. Biol. Pharm. Bull. 26(6) , 799-802 .

Takaya, A., Tomoyasu, T., Tokumitsu, A., Morioka, M. and Yamamoto, T. (2002) The ATP-dependent Lon protease of Salmonella enterica serovar Typhimurium regulates invasion and expression of genes carried on Salmonella pathogenicity island 1. J. Bacteriol. 184 , 224-232 .

Shimomura, S., Shigenobu, S., Morioka, M. and Ishikawa, H. (2002) An experimental validation of orphan genes of Buchnera, a symbiont of aphids. Biochem. Biophys. Res. Commun. 291, 263-267 .

Sasaki, T., Kubo, T. and Ishikawa, H. (2002) Interspecific transfer of Wolbachia between two lepidopteran insects expressing cytoplasmic incompatibility: a Wolbachia variant naturally infecting Cadra cautella causes male killing in Ephestia kuehniella. Genetics 162, 1313-1319 .

Shimomura, S., Shigenobu, S., Morioka, M. and Ishikawa, H. (2002) An experimental validation of orphan genes of Buchnera, a symbiont of aphids. Biochem. Biophys. Res. Commun. 292, 263-267.

Matsumoto, K., Morioka, M. and Ishikawa, H. (1999) Phosphocarrier proteins in an intracellular symbiotic bacterium of aphids. J. Biochem. 126, 578-583.

Morioka, M. and Ishikawa, H. (1998) Insect chaperonin 60: Symbionin. Methods Enzymol. 290, 181-193.

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その他

國枝武和 (2011) 環境応答性ゲノム 生物の科学・遺伝 65(6), 68-70.

Iijima, R.*, Kunieda, T.*, Yamaguchi, S.*, Kamigaki, H., Fujii-Taira, I., Sekimizu, K., Kubo, T., Natori, S. and Homma, K.J. (2008) The Extracellular Adenosine Deaminase Growth Factor, ADGF/CECR1, Plays a Role in Xenopus Embryogenesis via the Adenosine/P1 Receptor. J. Biol. Chem. 283(4), 2255-2264.
*: equal contribution.

Namiki, T., Niwa, R., Sakudoh, T., Shirai, K., Takeuchi, H. and, Kataoka, H. (2005) Cytochrome P450 CYP307A1/Spook: a regulator for ecdysone synthesis in insects. Biochem. Biophys. Res. Commun. 337, 367-374.

Nakata, A., Ito, T., Nagata, M., Hori, S. and Sekimizu, K. (2004) GRIP1τ, a novel PDZ domain-containing transcriptional activator, cooperates with the testis-specific transcription elongation factor SII-T1. Genes Cells. 9, 1125-1135 .

Aozasa, N., Kubo, T., Sekimizu, K. and Natori, S. (2002) Identification and characterization of a Drosophila homologue of ATBP. Insect Mol.Biol. 11 , 31-36 .

Aozasa, N., Shiraishi, H., Kobayashi, A., Sekimizu, K., Kubo, T. and Natori, S. (2001) Interaction between ATBP and DmUbc9 in the expression of the Sarcophaga lectin gene. Biochem. Biophys. Res. Commun. 286, 949-952.

Aozasa, N., Shiraishi, H., Nakanishi-Matsui, M., Kobayashi, A., Sekimizu, K., Kubo, T. and Natori, S. (2001) Activation of the Sarcophaga lectin gene promoter by (A + T)-stretch binding protein. Eur. J. Biochem. 268, 2506-2511.

Taira, Y., Kubo, T. and Natori, S. (2000) Participation of transcription elongation factor XSII-K1 in mesoderm-derived tissue development in Xenopus laevis. J. Biol. Chem. 275, 32011-32015.

Shimoaraiso, M., Nakanishi, T., Kubo, T. and Natori, S. (2000) Transcription elongation factor S-II confers yeast resistance to 6-azauracil by enhancing expression of the SSM1 gene. J. Biol. Chem. 275, 29623-29627.

Shiraishi, H., Kobayashi, A., Sakamoto, Y., Nonaka, T., Mitsui, Y., Aozasa, N., Kubo, T. and Natori, S. (2000) Molecular cloning and characterization of SRAM, a novel insect rel/ankyrin-family protein present in nuclei. J. Biochem. 127, 1127-1134.

Ito, T., Seldin, MF., Taketo, MM., Kubo, T. and Natori, S. (2000) Gene structure and chromosome mapping of mouse transcription elongation factor S-II (Tcea1). Gene 244, 55-63.

Miura, T., Kamikouchi, A., Sawata, M., Takeuchi, H., Natori, S., Kubo, T. and Matsumoto, T. (1999) Soldier caste-specific gene expression in the mandibular glands of Hodotermopsis japonica (Isoptera: termopsidae). Proc. Natl. Acad. Sci. USA. 96, 13874-13879.

Ito, T., Seldin, MF., Taketo, MM., Kubo, T. and Natori, S. (1998) Gene organization and chromosome mapping of the testis-specific S-II. Mamm. Genome 9, 915-917.

Taira, Y., Kubo, T. and Natori, S. (1998) Molecular cloning of cDNA and tissue-specific expression of the gene for SII-K1, a novel transcription elongation factor SII. Genes Cells. 3, 289-296.

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