P-1 Insulin Receptor Signaling in Kisspeptin-Expressing Cells is not Required for Puberty Onset or Reproductive Competency in Mice

Maggie Corr, Greg M. Anderson

P-2 Negative Fetal FSH/LH Regulation in Late Gestation is Associated with Declined Kisspeptins/KISS1R Expression in the Tuberal Hypothalamus

Fabien Guimiot, Lucie Chevrier, Sophie Dreux, Didier Chevenne, Alain Caraty, Anne Lise Delzoide, Nicolas de Roux

P-3 Prenatal Testosterone Decreases Co-expression of Insulin Receptors in KNDy Neurons in Adult Ewes

Cernea, M., Phillips, R., Padmanabhan, V.'' , Coolen, L.M. and Lehman, M.N.

P-4 The Effect of Perinatal Exposure to Low Level Ethinyl Oestradiol and a Cocktail of Endocrine Disrupting Pesticides on Kisspeptin Neurons in the Rat Hypothalamus

Overgaard, A.; Holst, K.K., Mandrup, K.; Boberg, J.; Jacobsen, P.R.; Christiansen, S.; Hass, U.; Mikkelsen, J.D.

P-5 Endocrine Disruptors, RF-amides and Puberty

Sandra M, Losa-Ward, Katherine A McCaffrey, Heather B Patisaul,

P-6 Disappearance of the Proestrous LH Surge Caused by Decline in the Activity of Kisspeptin Neurons in Middle-age Female Rats

Misawa Niki Ishii, Kiyoshi Matsumoto, Hisanori Matsui, Nobuyuki Seki, Hirokazu Matsumoto, Kaori Ishikawa, Fumio Chatani, Gen Watanabe, Kazuyoshi Taya

P-7 Ovarian Phenotypes of Mouse Models of Heterozygous and Homozygous Deficiency of Gpr54: Evidence for a Direct Role of Kisspeptin Signaling in Ovarian Physiology?

F. Gaytan, D. García-Galiano, S. León, A. Romero-Ruiz, L. Pinilla, M. Tena-Sempere

P-8 Evidence for Indirect Regulatory Actions of the Puberty-Modulating Peptide, Nesfatin-, on the Kiss1 system: Role in the Gonadotropic Effect of Nesfatin-

D. Garcia-Galiano, M. Wu, F. Ruiz-Pino, L. Pinilla, M. Alreja, V.M. Navarro, M. Tena-Sempere

P-9 Neuroproteomic Approaches to Identify Novel Kisspeptin-Regulated Pathways in the Hypothalamus: Studies in Kiss1 KO Mice

A. Romero-Ruiz, L. Steffani, S. Leon, M. Manfredi, D. Garcia-Galiano, L. Pinilla, W.H. Colledge, M. Tena-Sempere,

P-10 Sexual Difference in the Phenotypical Characteristics of the Mouse Kisspeptin Neurones

Kalló, I., Hrabovszky, E.; Molnár,, C.S.; Caraty, A.; Ciofi, P.; Coen, C.W.; Liposits, Z., Vida, B.

P-11 Embryonic Expression and Function of Kisspeptins and their Receptors in a Teleost Fish, medaka

Okubo, K.; Hodne, K.; Kanda, S.; Shimada, H.; Oka, Y.; Weltzien, F.A.,

P-12 Ghrelin does not Act Directly on Kisspeptin Neurons to Suppress the Reproductive System

Smith, J.T.; Reichenbach, A.; Lemus M.; Andrews, Z.B.

P-13 Delayed Puberty in Pre- and Postnatally Glucocorticoid Administered Female Rats Occurs Independently of the Hypothalamic Kiss1-Kiss1r-GnRH system

Takeshi Iwasa, Toshiya Matsuzaki, Riyo Kinouchi, Ganbat Gereltsetseg, Hiroshi Nakazawa and Minoru Irahara

P-14 Direct Projection and Effect of Kisspeptin Neuron to the Tuberofundibular Dopaminergic Neuron in the Arcuate Nucleus of Rat Brain.

Nobuhiko Sawai, Norio Iijima, Hitoshi Ozawa, Toshiyuki Matsuzaki

P-15 Kiss’n with the Lights on

Javier A Tello, Rafael Pineda, Valery Grinevich, Gareth Leng, Robert P Millar,

P-16 Cloning of the Rat Kisspeptin Promoter Reveals Genome Database Misassembly: a Prelude to Creating Transgenic Rats and Investigating the Regulation of Kisspeptin Gene Expression

Pineda R, Tello AJ, Millar RP

P-17 Cloning of Goat TAC3 Upstream Region and Evaluation of its Promoter Activity

Suetomi, Y.; Matsuda, F.; Uenoyama, Y.; Tsukamura, H.; Maeda, K.-I.; Ohkura, S.

P-18 Comparison of the Efficacy of Intravenous Treatment of Kisspeptin and GnRH on Ovarian Activity in Japanese Black Beef Cows

Naniwa, Y., Nakatsukasa, K.; Setsuda, S.; Oishi, S.; Fujii, N.; Wakabayashi, Y.; Okamura, H.; Uenoyama, Y.; Tsukamura, H.; Maeda, K.-I.; Matsuda, F.; Ohkura, S.

P-19 Kisspeptin Neurons in the Medial Preoptic Area of Castrated Male Goats Mediate the Surge-like Luteinizing Hormone Secretion Induced by Acute Elevations in Circulating Estrogen

Nakatsukasa, K.; Suetomi, Y.; Naniwa, Y.; Ito, D.; Inoue, N.; Wakabayashi, Y.; Okamura, H.; Maeda, K.-I.; Uenoyama, Y.; Tsukamura, H.; Matsuda, F.; Ohkura, S.

P-20 Generation of Immortalized Neuronal Cell Lines Derived from Goat Hypothalamic Arcuate Nucleus and Medial Preoptic Area

Matsuda, F.; Suetomi, Y.; Uenoyama, Y.; Maeda, K.-I.; Tsukamura, H.; Ohkura, S.

P-21 Corticotropin-Releasing Factor Receptor and Glucocorticoid Receptor Immunoreactivities in Kisspeptin Neurons in Female Rat Hypothalamus

Takumi, K.; Iijima, N.; Ozawa, H.

P-22 Kisspeptin Inputs to Hypothalamic Tuberoinfundibular Dopaminergic Neurons from Neonate to Puberty in Female Rats by Immunohistochemical Analysis

Iwata, K., Ikehara, M. , Iwatate, H., Suga, M., Kobayashi, A., Shirai, S., Aikawa, S., Matsumoto, K., Iijima, N., Ozawa, H.

P-23 Regulation of kiss2 gene by Thyroid Hormone in the Tilapia

Ogawa, S.; Ng K.W., Xue X.Y., Liu X.C., Lin H.R.; Parhar, I.S.

P-24 Neonatal Exposure to Dexamethasone Alter Reproductive Maturation and Kisspeptin Expression in Female Mice

Sandun L. Dalpatadu, Tomoko Soga, Ishwar S. Parhar

P-25 Regulation of Kiss1 Gene Expression in the Hypothalamus of Female Hatano Rats

Horii, Y, Dalpatadu, S.L., Soga, T, Ohta, R, Watanabe, G, Taya, K, Parhar, I.S.

P-26 Involvement of Kisspeptin Neurons in the Regulation of Estrogen-Induced Luteinizing Hormone Surge in both Sexes of the Japanese macaque (Macaca fuscata fuscata).

Watanabe, Y.; Uenoyama, Y.; Ieda, N.; Kawahara, M.; Takase, K. ; Suetomi, Y. ; Suzuki, J.; Ohkura, S.; Maeda, K.-I; Tsukamura, H.

P-27 Hypothalamic KISS1 Expression is not Associated with Onset of Puberty in Pigs

Ieda, N.; Uenoyama, Y.; Tomikawa, J.; Inoue, N.; Matsuda, F.; Ohkura, S.; Maeda, K.-I. and Tsukamura, H.

P-28 Kappa-opioid Receptor Partially Mediates Estrogen-Negative Feedback Effect on LH Pulses

Mostari, M. P.; Ieda, N.; Deura, C.; Minabe, S.; Uenoyama, Y.; Maeda, K.-I.; Tsukamura, H.

P-29 Analysis of Luteinizing Hormone Pulse and Surge in Female Mice

Minabe, S., ; Deura, C.; Uenoyama, Y.; Maeda, K.-I.; Tsukamura, H.

P-30 Kisspeptin Neurons Mediate Excitatory Amino acid-induced Luteinizing Hormone Secretion in Rats

Ikegami, K.; Uenoyama, Y.; Nakamura, S.; Tomikawa, J; Goto, T., Sambo, M.; Tamura, C., Hirabayashi, M.; Maeda, K.-I.; Tsukamura, H.

P-31 Establishment of Primary Kiss1 Neuron Culture for the Analysis of Gonadotropin-Releasing Hormone Pulse Generating System using Kiss1-GFP Transgenic Mouse

Abe, H.; Haruta, T.; Goto, T.; Fukanuma, T.; Takase, K.; Sambo, M. ; Tomita, K. ; Hirabayashi, M.; Imamura, T. ; Tomikawa, J. ; Uenoyama, Y.; Maeda, K.-I. ; Tsukamura, H.

P-32 Kiss1 Knockout Rats to define role of Kisspeptin in regulating Luteinizing hormone secretion

Uenoyama, Y.; Nakamura, S.; Hayakawa, Y.; Minabe, S.; Tomikawa, J.; Goto, T., Ieda, N., Sanbo, M., Tamura, C., Hirabayashi, M., Maeda, K.-I.; Tsukamura, H.

P-33 Kiss1 Gene is Required for Defeminization of the Brain Controlling Sexual Behaviors in Rats

Nakamura, S.; Uenoyama, Y.; Ikegami, K.; Tomikawa, J.; Goto, T., Sanbo, M.; Tamura, C., Hirabayashi, M.; Maeda, K.-I.; Tsukamura, H.

P-34 Kiss1 Upstream Region Regultes Hypothalamic Arcuate Nucleus-Specific Kisspeptin Expression

Goto, T., Tomikawa, J.; Abe, H.; Fukanuma, T.; Imamura, T.; Takase, K. ; Sanbo, M.; Tomita, K.; Hirabayashi, M.; Tsukamura, H.; Maeda, K.-I.; Uenoyama, Y.

P-35 Regional Distributions of Kisspeptin and Gonadotropin-Inhibiting Hormone (GnIH) Peptides in Ovine and Rat Brains

Hasegawa, H. ; Hashimoto, O.; Tsutsui, K.

P-36 Overexpression of KISS1R Induces Invasiveness of Estrogen Receptorα-Negative Human Mammary Epithelial and Breast Cancer Cells Lines

Cvetković, D., Dragan, M., Pape, C., Mir, Z., Leith, S.J., Leong. H.S., Pampillo, M , Babwah, A.V. , Bhattacharya, M.

P-37 Single-Cell Analyses Reveal that KISS1R-expressing Cells Undergo Sustained Kisspeptin-Induced Signaling that is Dependent upon an Influx of Extracellular Ca²⁺

Babwah AV, Pampillo M, Min L, Kaiser UB, Bhattacharya M

P-38 Regulation of the Oxytocin System by Central Kisspeptin

Scott, V.; Seymour, A.J.; Campbell, R.E.; Brown, C.H.

P-39 Evidence for a Concentration-Dependent Reduction of GPR54 Response after Repeated Stimulation by Kisspeptin

M. Beltramo, V. Robert, N. De Roux, F. Foulon-Gauze, A. Caraty

P-40 GnRH Release into the Hypophyseal Portal Blood of the Ewe Mirrors both Pulsatile and Continuous Intravenous Infusion of Kisspeptin: Insight into Kisspeptin Mechanism of Action.

A. Caraty, D. Lomet, Sébert ME, D Guillaume, M. Beltramo & NP Evans

P-41 Kisspeptin Neurons are Activated during the Male Effect in Sheep

Ghenim M, S. ; Dufourny, L. ; Fabre-Nys, C.

P-42 Kisspeptin Regulation of Arcuate Neuron Excitability in Gpr54 Knockout Mice

Xinhuai Liu & Allan E. Herbison

P-43 Alterations in Neural Kiss1 Expression in Adult GABAB Receptor Knockout Mice

Noelia P Di Giorgio, Paula V López, Sheila J Semaan, Bernhard Bettler, Carlos Libertun, Victoria A Lux-Lantos, and Alexander S Kauffman

P-44 Investigating the Possible Interconnectivity of the Kisspeptin and RFamide-Related Peptide 3 (RFRP-3) Systems in Adult Mice

Matthew C. Poling, and Alexander S. Kauffman

P-45 Impaired Kisspeptin Signaling Induces an Obese and Diabetic Phenotype in Adult Mice

Kristen P. Tolson^*, Jeremy T. Smith^*, Stephanie Yen, Stephanie Simmonds, Aneta Stefanidis, and Alexander S. Kauffman

P-46 Disruption of Glucocorticoid Receptor Signaling in Kisspeptin Neurons Accelerates the Recovery of Reproductive Function after Stress

Oulu Wang, , Anne Lanjuin, Caroline Ho, Juliana Basko-Plluska, Louis Muglia, Catherine Dulac, Joseph A. Majzoub,

P-47 Circulating Kisspeptin and Pituitary Adenylate Cyclase-Activating Polypeptide Do Not Corelate with Gonadotropin Serum Levels

Kanasaki, H. , Oride, A. , Mijiddorj, T. , Sukhbaatar, U. , Miyazaki, K.

P-48 Arcuate Kisspeptin Neurones Play a Physiological Role in the Control of the Oestrous Cycle in Adult Female Rats

Kinsey-Jones JS, Beale KE, Gardiner JV, Harrison EK, Thompson EL, Sleeth ML, Sam AH, Dhillo WS, Bloom SR, and Murphy KG

P-49 Sex-Related Differences in the Effect of RFRP-3 on the Gonadotrophic Axis in the Syrian Hamster

Ancel, C., Tena-Sempere, M., Mikkelsen, J.D., Simonneaux, V.

P-50 Interactions between Neuropeptide Y and Neurokinin B in Kiss1 Neurons as a Link between Metabolism and Reproduction.

Wu, M., Steiner, R.A. and Alreja, M.,

P-51 Dynorphin Inhibits KNDy Neurons but not GnRH Neurons

Wu, M. and Alreja, M.,

P-52 Structure-Activity Relationship Study of Tachykinin Peptides for Development of Novel Neurokinin-3 Receptor Agonists

Misu, R.; Oishi, S.; Noguchi, T.; Yamada, A.; Ohno, H.; Yamamura, T.; Okamura, H.; Matsuda, F.; Ohkura, S.; Fujii, N.

P-53 Exploring the Roles of Kisspeptin Partners, NKB and Dynorphin, in the Control of the Gonadotropic Axis: Shaping the KNDy Paradigm

F. Ruiz-Pino, D. Garcia-Galiano, M. Manfredi, S. Leon, M.A. Sanchez-Garrido, J. Roa, L. Pinilla, M. Tena-Sempere, V.M. Navarro,

P-54 CRF and AVP Are Not Involved in the Neurokinin B/NK3R-Mediated Suppression of the GnRH Pulse Generator in the Ovariectomized Rat

Pasha Grachev, Xiao Feng Li, James Kinsey-Jones, Yuanshao Lin, Shengyun Li, Minghan Hu, Robert Millar, Stafford Lightman, Kevin O'Byrne

P-55 Continuous Administration of Senktide, a Neurokinin B Receptor Agonist, Facilitates Intermittent Activities of the GnRH Pulse Generator for 24h in Goats

Yamamura, T.; Wakabayashi, Y.; Noguchi, T.; Oishi, S.; Fujii, N.; Ohkura, S.; Okamura, H.

P-56 Neuroanatomical Distribution of Kisspeptin systems in the Brain of the Perciform Scombroid fish (Scomber japonicus)

Selvaraj, S., Kitano, H., Ohga, H., Nyuji, M., Yamaguchi, A., Matsuyama, M.

P-57 Identification and Seasonal Expression Variations of Two Subtypes of Kisspeptin Receptors in a Scombroid Fish, Chub mackerel

Ohga, H., Selvaraj, S., Fujinaga, Y., Kitano, H., Nyuji, M., Yamaguchi, A., Matsuyama, M.

P-58 Expression Profiles of kiss2, gpr-54-2, and gnrh1 in Male and Female Japanese Anchovy (Engraulis japonicus) during Puberty

Sukhan, Z.P., Kawamura, K., Kitano, H., Selvaraj, S., Yamaguchi, A., Matsuyama, M

P-59 The Distribution of Kisspeptin and Gonadotrophin-Releasing Hormone (GnRH) Neurons in the Hypothalamus of the Dog

Scott, C.J., Buchholz, V., Norman, S.T.

P-60 Electrophysiological and Morphological Analysis of Kiss1 Neurons in Trasgenic Medaka, Oryzias latipes

Shimada, H.; Kanda, S.; Akazome, Y.; Abe, H.; Okubo, K.; Oka, Y.

P-61 Effects of Kisspeptins on LH Secretion and GnRH1 Neuronal Activities in Goldfish and Medaka.

Karigo, T.; Uenoyama, Y.; Oishi, S.; Fujii, N.; Mitani, Y. ; Kanda, S.; Oka, Y.

P-62 Generation of kiss1 Knockout Medaka using Efficient New Genetic Tools, TALENs

Takahashi, A.; Kanda, S.; Akazome, Y.; Oka, Y.

P-63 Steroid Sensitive Hypothalamic Kiss1 Neurons in Medaka do not Co-express NKB or DYN

Zempo, B.; Kanda, S.; Akazome, Y.; Oka, Y.

P-64 Kiss2 may Activate Receptors for Neuropeptide FF (NPFF) / RF Amide Related Peptide (RFRP) in Medaka

Akazome, Y.; Yamamoto, E; Oka, Y.

P-65 Inhibitory Regulation of GnRH3 Neurons by RFRP Neurons in Medaka

Nakane, R.; Kanda, S.; Oka, Y.

P-66 Interactions between Kisspeptin and Gonadotrophin in the Equine Hypothalamus

McGrath, B.M ; Norman, S.T ; Caraty, A.; Scott, C.J

P-67 Kiss1 Expression in the Rat Placenta: Changes with Gestational Age and Regulation by Glucocorticoids

Lewis, J.L.; Jones, M.L.; Mark, P.J.; Waddell, B.J.; Smith, J.T.

P-68 Influence of Kisspeptin on Lipid Metabolism and Endocrine Function of Adipocytes Isolated from Obese and Diabetic Rats.

J.H. Sliwowska, E. Pruszyńska-Oszmałek, P. Kołodziejski and P. Kaczmarek

P-69 An Epidemiological Hypothesis: Kisspeptin may Play an Important Role in the Protective Effects on Mortality and Epilepsy after Traumatic Brain Injury

Liao, C.C.; Chen T. L.

P-70 Proline-Arginine-Arginine Repeats in the Intracellular Domain of the Human KISS1R are Important for the Transduction Pathway and the Intracellular Export to Cell Membrane

Lucie Chevrier, Alexandre de Brevern, Eva Hernandez, Jérôme Leprince, Hubert Vaudry, Anne Marie Guedj, Nicolas de Roux

P-71 Investigating Plasma Kisspeptin in a Range of Human Cancers Reveals Clinical Utility in Lung and Ovarian Cancers

Comninos, A.N.; Jayasena, C.N.; Januszewski, A.; Gabra, H.; Taylor, A.; Harvey, R.A.; Ghatei, M.A.; Bloom, S.R.; Dhillo, W.S.

P-72 Effects of Neurokinin B Administration on Reproductive Hormone Secretion in Healthy Men

Channa N Jayasena, Akila De Silva, Gurjinder MK Nijher, Johannes D. Veldhuis, Alexander N Comninos, Ali Abbara, Meriel Vaal, Gordon Stamp, Mohammad A Ghatei, Stephen R Bloom, Waljit S Dhillo

P-73 The Relationship between Plasma Kisspeptin Kevels and Blood Pressure during Pregnancy

A Abbara, CN Jayasena, R Ratnasabapathy, C Otti, GM Nijher, L Sriskanadarajah, MK Dhanjal, MA Ghatei, SR Bloom, WS Dhillo

P-74 Plasma Kisspeptin Levels are Elevated in Boys and Girls when Compared with non-pregnant Adults

Risheka Ratnasabapathy, Gurjinder MK Nijher, Channa N Jayasena, Akila De Silva, Ali Abbara, Nicola Bridges, Mohammad A Ghatei, Stephen R Bloom, Waljit S Dhillo

P-75 Synthesis and Biological Evaluation of a Novel Nonapeptide Kiss1r Agonist with Testosterone-Suppresive Activity

Asami, T.; Nishizawa, N.; Matsui, H.; Ishibashi, Y.; Nishibori, K.; Horikoshi, Y.; Nakayama, M.; Matsumoto, S.; Tarui, N.; Yamaguchi, M.; Matsumoto, H.; Ohtaki, T.; Kitada, C.

P-76 Inhibitory Effect of Kisspeptin on Trophoblast Invasion / Migration in vivo

Renju S. Raj M.D., Natalia I. Gokina Ph.D., George Osol Ph.D.

P-77 Lack of Kisspeptin Responses in Hypogonadotropic Patients Who Exhibit Endogenous GnRH Neuronal Activity

Chan, Y.-M., Sidhoum, V.F., Seminara, S.B.