黒田研公開ラボセミナー
Encoding and decoding dynamic cell signaling: how to build a blood vessel
Dr. Andre Levchenko（Department of Biomedical Engineering and Systems Biology Institute, Yale University）
2018年08月06日(月) 10:00-11:30 理学部3号館 412号室
Dynamics of cell signaling can carry important information about the environment and the potential responses to changes in this environment. Furthermore, a single extracellular stimulus can promote diverse behaviors among isogenic cells by differentially regulated signaling networks. We examined Ca(2+) signaling in response to VEGF (vascular endothelial growth factor), a growth factor that can stimulate different behaviors in endothelial cells. We found that altering the amount of VEGF signaling in endothelial cells by stimulating them with different VEGF concentrations triggered distinct and mutually exclusive dynamic Ca(2+) signaling responses that correlated with different cellular behaviors. These behaviors were cell proliferation involving the transcription factor NFAT (nuclear factor of activated T cells) and cell migration involving MLCK (myosin light chain kinase). Further analysis suggested that this signal decoding was robust to the noisy nature of the signal input. Ca(2+) signaling patterns associated with proliferation and migration were detected during angiogenesis in developing zebrafish. In this talk, I will analyze how the signaling in the network can be balanced through diverse Ca(2+) dynamics, and how this dynamics can be robustly decoded and converted into distinct behaviors needed to build a functional blood vessel.
